Capsicum annuum L extracts modulate cell proliferation and intracellular Ca² signaling in human mammary epithelial cells
DOI:
https://doi.org/10.56162/transdigital610Keywords:
intracellular calcium signaling, human mammary cells, cell proliferation, functional foods, capsicum annuum LAbstract
This study evaluated the effect of extracts from three varieties of Capsicum annuum L on cellular metabolic activity and intracellular Ca²? signaling in human breast epithelial cells, using a tumor cell line (MCF-7) and a non-tumor cell line (MCF-12F). Metabolic activity was determined using the Alamar Blue assay, while Ca²? dynamics were analyzed using the Fluo-4 AM fluorescent indicator and real-time functional microscopy. The plant extracts produced differential effects depending on cell type and fruit variety. More pronounced changes in metabolic activity were observed in MCF-7 cells, while in MCF-12F cells, transient increases in intracellular Ca²? were recorded with distinct kinetic patterns among varieties. The results indicated that complex phytochemical matrices derived from Capsicum annuum L interact with the Ca²? signaling machinery and can modify cellular physiology in a tumor-context-dependent manner. These findings provide experimental evidence regarding the interaction between plant-derived compounds and fundamental cellular pathways related to human health.
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Copyright (c) 2026 Verónica Morales-Tlalpan, Roberto Jorge García Mendoza, Carlos Saldaña Gutiérrez, Ana Angélica Feregrino Pérez
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